Fossa mating behavior is unusual among carnivores. Breeding is seasonal, typically in the southern-hemisphere spring, and often centers on a mating tree. A female occupies a site high in a tree, and several males gather below and compete over the days that follow for the chance to mate. Copulation frequently takes place up in the branches and can last a long time by mammalian standards.
Gestation lasts about 90 days. Females usually give birth to two to four young, called pups, in a den such as a tree hollow, rock cavity, or burrow. Pups are born small and helpless, weighing around 100 grams, and their eyes are closed at first. They are weaned at roughly four to four and a half months but develop slowly, staying with the mother and not becoming fully independent until somewhere between 15 and 20 months of age. Wild lifespan is poorly documented, while captive fossas have lived up to about 20 years.